Figure 2.

Cellular functions of the combinatorial antibody ligands. a) Western‐blot of STAT3 and phospho‐STAT3 in LepR‐expressing HEK293 cells treated with/without 10 × 10⁻⁹ m leptin, H6, Ab06, and Ab11, respectively. b) Dose‐dependent activation of LepR by leptin and combinatorial antibody ligands Ab06, Ab08, Ab11, and H6 via chemiluminescent detection (luminescence) of STAT3 phosphorylation in LepR or non‐LepR expressed luciferase reporter cell lines. Data are from three independent experiments and shown as means ± SEM (error bars). c) Dose‐dependent activation of LepR by leptin and combinatorial antibody ligands Ab06, Ab08, Ab11, and H6 via leptin‐dependent cell proliferation of murine IL‐3‐dependent LepR expressed or non‐LepR expressed Ba/F3 pre‐B cells (OD490, absorption at wavelength 490 nm). Data are from three independent experiments and shown as means ± SEM (error bars).