Table 2.

Summary of kinetic constants of leptin and scFv antibody ligands in binding affinity measurement with LepR‐ECD. Data are displayed using scientific notation. Antibodies and leptin in the table represent the corresponding scFv‐Fc and leptin‐Fc format, respectively. Data were processed by BIA evaluation software and K _D values were calculated by fitting the curve in 1:1 binding model. All kinetic parameters (K _D, k _on, and k _off) are calculated by the average of five different ligand concentrations. N/A represents not applicable

Ligands	hLepR‐ECD			mLepR‐ECD
	K D [M]	k on [1 M−1 s−1]	k off [1 s−1]	K D [M]	K on [1 M−1 s−1]	k off [1 s−1]
Leptin	5.0 × 10−10	2.6 × 106	1.3 × 10−3	7.9 × 10−10	2.8 × 106	2.2 × 10−3
H6	3.9 × 10−10	7.9 × 105	3.1 × 10−4	1.7 × 10−8	2.1 × 106	3.5 × 10−2
Ab06	7.5 × 10−9	6.9 × 106	5.2 × 10−2	N/A	N/A	N/A
Ab11	2.4 × 10−8	4.7 × 105	1.1 × 10−2	2.0 × 10−7	9.6 × 104	1.9 × 10−2