Figure 4.

Evaluation of the immune abscopal effect for inhibiting the growth of distant tumors. a) A schematic illustration of the development of a bilateral 4T1 tumor model and strategies for phototherapy and immune analysis. b) Growth curves of distant tumors after the treatment for the primary tumor. c) Proportions of tumor‐infiltrating of CD45+ cells in the distant tumors after various therapies for the primary tumors. d) The representative flow cytometric analysis of tumor‐infiltrating T cells (CD4+ and CD8+) in distant tumors and their relative proportions in tumor tissues at 3, 7, and 14 days from different treatment groups. e) Representative immunofluorescence images of infiltrating T lymphocytes in the distant tumor at 14 days post‐treatment in different treatment groups. f) Recruitment of DCs into the distant tumor (left columns) and tumor‐draining lymph nodes (right columns) in different treatment groups at various time points after treatments. g,h) Proportions of DCs (Live CD45+CD11c+MCH II+CD80+CD86+) in the distant tumor (g) and tumor‐draining lymph nodes (h) at day 3, 7, and 14 after different phototherapy. i) Visualization and fluorescence images of long‐term migration of Gd‐Ce6@SWNHs to tumor‐draining lymph nodes at 24 h and 7‐day post PDT + PTT combination treatment. The lymph nodes were indicated by the circles; *p < 0.05; **p < 0.01.