Figure 4.

Long‐term culture of hNSCs in 3D‐printed devices: a) Brightfield image of the necrotic core identified in the F‐hNSC population 3 days after the differentiation was initiated in a conventional compartmentalized PDMS device. On day 5 of differentiation, the necrotic region expands throughout the whole population. b) Graphical illustration of a two‐compartment device used in the cell viability study. c) Comparison of PDMS content between the conventional device and the devices made using 3D printed soft lithography. d) A bar chart displaying the first recorded observation of the necrotic region for each hNSC line in compartmentalized devices with decreasing ratios between the cell media and the PDMS surface area exposed to the media (mean value with standard deviation). The insert shows live/dead staining of the cells after 40 days of differentiation in the device with PMMA extensions.