Mice lacking of cELV are hypotensive. a) Schematic timeline shows overall study approach in mice. Lentivirus was injected subcutaneously (SQ) into 2 week old mice. b) The survival curve shows that the PGRMC2 knockdown mice had a significantly higher mortality rate. Systolic (SBP, c), diastolic (DBP, d) blood pressure, mean arterial pressure (MAP, e), heart rate (HR, f) and body weight (g) were recorded from 6 8, and 10 week old (w.o.) mice after 1 week of acclimation. h) Representative electron micrographs indicate PGRMC2 and F11R knockdown mice lacked of cELV. Arrow indicates cELV in scrambled control mouse. i) Circulating cELVs were quantified by collecting serum of the mice at the end point of the study. N = 8–12 mice for the scrambled control, PGRMC2 and F11R knockdown groups. *, p < 0.05; **, p < 0.01; compared with the scrambled control.