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. 2020 Aug 18;10:13936. doi: 10.1038/s41598-020-70771-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intensities of CD47 staining of NK cells, CD8⁺ T cells, and nonlymphocytes in the tumoral and paratumoral compartments of EC patients. (A) The analyzed cells were gated as in Fig. 1A, and the mean fluorescence intensities of CD47 staining (CD47 MFI) of NK cells (CD45⁺CD3⁻CD56⁺), CD8⁺ T cells (CD45⁺CD3⁺CD8⁺), and nonlymphocytes (CD45⁻) in tumor tissue, peritumoral tissue and adjacent tissue samples from 12 EC patients were determined by flow cytometry. (B) The CD47 MFIs determined in (A) were evaluated within each tissue compartment. In (A) and (B), the significance of differences was determined by nonparametric one-way ANOVA with Dunn's multiple comparison test (n = 12). *P < 0.05 was considered significant. The data are shown as the mean ± SEM.