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. 2020 Aug 18;10:13936. doi: 10.1038/s41598-020-70771-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NK cell stimulation is not impacted by anti-CD47 antibody. (A) The gating strategy for flow cytometry analyses of CD107a externalization in NK cells (CD45⁺CD3⁻CD56⁺). PBMCs were treated or not treated with anti-CD47 antibody and stimulated with vehicle (Vehicle, top panels) or K-562 cells and IL-2 (K562/IL-2, middle panels). As a control, the target K-562 cells alone were analyzed (K562 only). (B) The gating strategy for flow cytometry analyses of TNFα intracellular staining in NK cells (CD45⁺CD3⁻CD56⁺). The samples as in (A) are show. (C) The data in (A) and (B) were evaluated and the significance of differences determined by Wilcoxon matched-pairs signed-ranks test (n = 6 donors). *P < 0.05 was considered significant.