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. 2020 Aug 12;11:988. doi: 10.3389/fphys.2020.00988

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Copyright © 2020 Shen, Zhang, Huang, Zhu, Qiu, Ma, Yang, Ding and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Isoquercitrin inhibits oxidative stress in denervated soleus muscles (n = 6). (A) Fluorescence diagram of reactive oxygen species (ROS) level and quantification of relative fluorescence intensity of each group in the dihydroethidium (DHE) probe detection experiment. (B) Quantitative polymerase chain reaction detection of the relative expression of antioxidant-related genes and ROS production-related genes. (C) Western blot of ROS-related and antioxidant-related proteins and quantification of the relative expressions. ROS, reactive oxygen species; Ctrl, control group; Den, denervation group; ISO, denervated target muscle plus isoquercitrin (20 mg/kg/d) group. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, and ^****p < 0.0001 vs. Ctrl; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, and ^####p < 0.0001 vs. Den.