REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
E. coprostanoligenes	American Type Culture Collection	ATCC51222
E.Coli	ThermoFisher	BL2- EC0114
Biological Samples
Stool culture	This paper	N/A
Chemicals, Peptides, and Recombinant Proteins
β-Nicotinamide adenine dinucleotide hydrate	Sigma	N6522-1G
β-NADH phosphate disodium salt	Sigma	10128040001
Coprostan-3-ol	Sigma	C7578-50MG
L-α-Phosphatidylcholine	Sigma	P3644-25G
Cholesterol	Sigma	C3045-5G
Sodium thioglycolate	Sigma	T0632-25G
Calcium Chloride, Dihydrate, Molecular Biology Grade - CAS 10035-04-8 – Calbiochem	Sigma	C5670-100g
Yeast Extract	Sigma	Y1625-250G
(+)-4-Cholesten-3-one (cholestenone)	Sigma	188174-10G
BD Difco Agar, Technical	Fisher Scientific	DF0812-17-9
cOmplete, EDTA-free Protease Inhibitor Cocktail	Sigma	11873580001
Lysonase Bioprocessing Reagent	Sigma	71230
Critical Commercial Assays
DNeasy PowerSoil Kit	Qiagen	12888
High-Capacity cDNA Reverse Transcription Kit	Applied Biosystems	4368813
iTaq Universal SYBR Green Supermix	BioRad	1725121
Deposited Data
Framingham Heart Study metagenomics data	This paper	BioProject PRJNA559860
Eubacterium coprostanoligenes genome	This paper	BioProject PRJNA559861
Stool Culture sequencing data	This paper	BioProject PRJNA559861
Oligonucleotides
Primers for qPCR of putative cholesterol oxidoreductases in E. coprostanoligenes, see Table S3	This paper	N/A
Primers for cloning putative cholesterol oxidoreductases into the pET28 vector, see Table S3	This paper	N/A
Primers for site directed mutagenesis of ECOP170, see Table S3	This paper	N/A
Recombinant DNA
pET28 plasmid	N/A	N/A
Geneblocks for the cholesterol oxidoreductases from human-associated bacteria, see Table S3	Genewiz	N/A
Software and Algorithms
Trim_Galore! (v0.4.4 )	Babraham Bioinformatics	https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/
Trimmomatic (v0.36)	Bolger et al., 2014	http://www.usadellab.org/cms/?page=trimmomatic
KneadData (v0.7.2)	Huttenhower lab	http://huttenhower.sph.harvard.edu/kneaddata
MegaHIT (v1.1.4)	Li et al., 2015	https://github.com/voutcn/megahit
Prodigal (v2.6.3)	Hyatt et al. 2010	https://github.com/hyattpd/prodigal/releases/
SPAdes (v3.9.0)	Bankevich et al. 2012	https://github.com/ablab/spades/releases
BWA (v0.7.17)	Li and Durbin, 2009	http://bio-bwa.sourceforge.net/
SAMTools (v1.8)	Li and Durbin, 2009	http://www.htslib.org/
CD-HIT (v4.7)	Fu et al. 2012	http://weizhongli-lab.org/cd-hit/
CheckM (v1.0.13)	Parks et al. 2015	https://github.com/Ecogenomics/CheckM/releases
PhyloPhlAn (v0.99)	Segata et al., 2013	https://huttenhower.sph.harvard.edu/phylophlan/
FastANI (v1.2)	Jain et al. 2018	N/A
EggNOG Mapper (v1.0.3)	Huerta-Cepas et al., 2016, Huerta-Cepas et al., 2019	http://eggnog-mapper.embl.de/
USEARCH (v8.1)	Edgar, 2010	https://www.drive5.com/usearch/
BLAST+ (v2.6.0+)	NCBI	https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastDocs&DOC_TYPE=Download
MSPminer	Plaza Oñate et al., 2019	https://www.enterome.com/downloads/
R (v3.6.1)	packages for analytical part: stats, nlme, lme4, meta	https://www.r-project.org/