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. 2020 Jul 24;25(15):3365. doi: 10.3390/molecules25153365

Figure 2.

Figure 2

Screening of peptidomimetics against the FGF14:Nav1.6 complex using the split luciferase complementation assay (LCA). (A) Clone V cells were treated with compounds (25 μM; n = 8 wells per compound) in 96-well plates for 12 h prior to luminescence reading, and maximal luminescence for each well was normalized to per plate 0.5% DMSO controls (n = 32 per plate). (B) To assess effects of compounds on cell viability, the CellTiter Blue reagent was dispensed immediately following luminescence reading in (A). Fluorescence was detected after incubation for 18 h. Tamoxifen, known to be toxic to HEK293 cells [33], was used as a positive control. Individual replicate values with mean ± standard error of the mean (SEM) are shown. Significance was assessed using one-way ANOVA with post hoc Dunnett’s multiple comparisons test. ** p < 0.0005. * p < 0.005.