Figure 3.

Dose response curves of top FGF14:Nav1.6 complex modulators. To determine potency (IC₅₀ or EC₅₀) and efficacy (percent luminescence at the bottom or top plateau for inhibitors (I_Min) or enhances (E_Max), respectively) each compound was tested at 14 concentrations (range: 0.25–175 µM; n = 4 replicates per concentration) using the LCA in 96-well plates. Data are shown as mean percent luminescence (relative to per plate 0.5% DMSO controls) values ± SEM. Non-linear regression curve fitting was performed using GraphPad Prism 8 to determine potency and efficacy values for each compound, as shown in Table 2.