Table 4.

Functional modulation of Nav1.6 by 12 and 19 relative to control ^a.

Condition	Peak Current Density (pA/pF) b	Tau of Fast Inactivation (ms) c	V1/2 of Activation (mV) d	V1/2 of Steady-state Inactivation (mV) e	Long Term Inactivation (Depolarization Cycles 2–4) f
					2	3	4
DMSO	–55.39 ± 2.16 (10)	1.17 ± 0.09 (7)	–20.04 ± 0.85 (10)	–58.58 ± 1.90 (5)	0.89 ± 0.03	0.86 ± 0.03	0.85 ± 0.04 (6)
12	–18.30 ± 2.02 ** (10)	1.30 ± 0.11 (7)	–20.40 ± 0.89 (10)	–57.59 ± 0.56 (6)	0.88 ± 0.03	0.76 ± 0.03 *	0.70 ± 0.04 * (7)
19	–79.63 ± 3.55 ** (8)	1.12 ± 0.07 (7)	–22.49 ± 0.72 * (8)	–58.53 ± 1.69 (8)	0.83 ± 0.02	0.73 ± 0.02 *	0.73 ± 0.03 * (8)

^a Summary of the electrophysiological evaluation of 12 and 19. Results are expressed as the mean ± SEM. The number of independent experiments is shown in parentheses. Significance was determined via unpaired t-test compared with 0.1% DMSO. * p < 0.05; ** p < 0.005. ^b Peak current density, which describes the number of channels in a conductive (open) state, is a measure of the maximum influx of Na⁺ current (pA) into the cell normalized to membrane capacitance (pF) to control for variable cell sizes. ^c Tau of fast inactivation measures the decay phase of Na⁺ currents to characterize the time required for channels to transition from the conductive (open) state to a nonconductive state resulting from fast inactivation. ^d V_1/2 of activation is a measure of the voltage at which half of available channels transition from the closed to the conductive (open) state. ^e V_1/2 of steady-state inactivation is a measure of the voltage at which half of channels are available to transition into the conductive (open) state, while the other half are non-conductive due to steady-state (closed-state) inactivation. ^f Long-term (slow) inactivation is a type of inactivation induced by successive or prolonged depolarization cycles that causes collapse of the S5–S6 pore module of Na_v channels resulting in a prolonged nonconductive inactivated state.