Table 3.

Eco-scale evaluation of the developed method.

Chemical Compound Score			Sub-Total PP	Total PP
Ethanol	Amount	<10–100 mL	2	2
	Hazard	Less severe hazard	1
Water	Amount	<10–100 mL	2	0
	Hazard	none	0
Acetic acid	Amount	<10 mL	1	1
	Hazard	1 pictogram-warming	1
				∑ = 3
Instrument score
				Total PP
Energy		≤0.1 kWh per sample		0
Occupational hazard		Analytical process hermetization		0
Waste	1–10 mL			3
				∑ = 3
Total penalty points (PP): 6
Analytical Eco-scale score: 100 − 6 = 94