Table 1.
Content (mg/100 g d.w.) and composition of tentatively identified polyphenols in wild Brassica spp. used in this investigation.
| # | UV λmax (nm) | [M + H]+/ [M − H]- | MS2 (m/z) (%) | Compound a | B.incana | B. macrocarpa | B. rupestris | B. villosa | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BN | BW | BB | BU5 | BU9 | BU15 | BL | BX | BY5 | DC | ||||||||
| 1 | 235, 263, 319 | 951/949 | 625(100); 301(5) | Q-3-O-diglucoside- 7-O-diglucoside |
2.3 ± 0.0 | 9.6 ± 0.1 | 18.1 ± 0.1 | 6.0 ± 0.4 | 69.2 ± 10.7 | 2.5 ± 0.02 | 13.0 ± 0.2 | 3.6 ± 0.3 | 5.7 ± 0.6 | 6.6 ± 0.0 | |||
| 2 | 239, 263, 338 | 935/933 | 701(100); 285(2) | K-3-O-triglucoside- 7-O-glucoside |
51.2 ± 1.9 | 3.3 ± 0.5 | 23.7 ± 6.6 | - | - | - | 50.2 ± 3.1 | 46.9 ± 2.7 | 80.4 ± 8.6 | 66.9 ± 3.1 | |||
| 3 | 237, 255, 330 | 1275/1273 | 1111(100); 949(43); 787(68); 301(1) | Q-3-O-caffeoyltriglucoside- 7-O-diglucoside |
- | - | - | 15.1 ± 1.1 | 59.8 ± 9.1 | 18.8 ± 7.4 | - | - | - | - | |||
| 4 | 239, 267, 331 | 1127/1125 | 801(100); 609(27); 285(1) | K-3-O-hydroxyferuloyl diglucoside-7-O-diglucoside |
52.3 ± 1.9 | 99.7 ± 1.3 | - | - | - | - | - | - | - | - | |||
| 5 | 235, 263, 330 | 935/933 | 609(100);285(2) | K-3-O-diglucoside- 7-O-diglucoside |
- | - | 28.1 ± 1.9 | - | - | - | 52.9 ± 3.0 | 36.0 ± 2.2 | 16.3 ± 2.0 | 50.1 ± 1.3 | |||
| 6 | 239, 267, 331 | 995/993 | 831(100); 787(88); 625(56); 301(1) | Q-3-O-sinapoyldiglucoside- 7-O-glucoside |
- | - | - | - | - | - | 16.5 ± 0.5 | 9.1 ± 1.2 | 21.2 ± 2.1 | 30.7 ± 0.1 | |||
| 7 | 243, 267, 331 | 1097/1095 | 771(100); 609(17) | K-3-O-caffeoyldiglucoside- 7-O-diglucoside |
62.5 ± 2.1 | 43.5 ± 2.2 | - | - | - | - | - | - | - | - | |||
| 8 | 245, 340 | 1319/1317 | 1111(100); 993(72); 787(87); 301(2) | Q-3-O-sinapoyltriglucoside- 7-O-diglucoside |
- | - | - | 54.0 ± 2.3 | 73.7 ± 9.6 | 12.5 ± 0.0 | - | - | - | - | |||
| 9 | 237, 339 | 1157/1155 | 949(100); 831(32); 625(35); 301(1) | Q-3-O-sinapoyldiglucoside- 7-O-diglucoside |
- | - | - | - | - | - | 76.1 ± 2.4 | 38.9 ± 2.4 | 17.7 ± 2.6 | 11.6 ± 0.5 | |||
| 10 | 235, 340 | 951/949 | 787(100); 625(52); 301(18) | Q-3-O-caffeoyldiglucoside- 7-O-glucoside |
- | - | 9.2 ± 0.6 | - | - | - | - | - | - | - | |||
| 11 | 253, 347 | 1289/1287 | 1111(93); 963(75); 787(100); 301(1) | K-3-O-feruloyltriglucoside- 7-O-diglucoside |
- | - | - | 45.4 ± 12.3 | 62.1 ± 9.3 | 74.8 ± 0.2 | - | - | - | - | |||
| 12 | 239, 267, 331 | 979/977 | 815(100); 771(2); 609(7); 285(2) | K-3-O-sinapoyldiglucoside- 7-O-glucoside |
27.7 ± 0.3 | 28.2 ± 0.7 | 7.0 ± 0.1 | - | - | - | 40.0 ± 1.3 | 86.1 ± 6.1 | 24.8 ± 3.7 | 31.3 ± 0.6 | |||
| 13 | 239, 267, 330 | 1303/1301 | 1095(7); 977(100); 771(75); 285(2) | K-3-O-sinapoyltriglucoside- 7-O-diglucoside |
- | - | - | 62.0 ± 2.2 | 19.5 ± 2.9 | 36.7 ± 0.4 | - | - | - | - | |||
| 14 | 243, 267, 331 | 1141/1139 | 815(100); 609(28); 285(1) | K-3-O-sinapoyldiglucoside- 7-O-diglucoside |
81.3 ± 1.8 | 67.5 ± 0.7 | 7.7 ± 0.4 | - | - | - | 27.1 ± 0.9 | 81.4 ± 4.4 | 37.1 ± 3.9 | 25.8 ± 1.3 | |||
| 15 | 239, 264, 331 | 949/947 | 785(100); 609(7); 285(2) | K-3-O-feruloyldiglucoside- 7-O-glucoside |
- | - | - | - | - | - | 21.5 ± 0.6 | 10.1 ± 1.2 | 43.3 ± 4.2 | 32.1 ± 0.1 | |||
| 16 | 239, 267, 331 | 1111/1109 | 785(100); 609(21); 285(1) | K-3-O-feruloyldiglucoside- 7-O-diglucoside |
38.5 ± 1.1 | 25.3 ± 1.5 | - | - | - | - | 34.3 ± 1.5 | 30.9 ± 1.9 | 30.6 ± 3.0 | 42.3 ± 0.2 | |||
| 17 | 239, 265, 332 | 1273/1271 | 1095(10); 947(100); 771(64); 285(3) | K-3-O-feruloyltriglucoside- 7-O-diglucoside |
- | - | - | 19.5 ± 0.1 | 26.2 ± 4.0 | 16.7 ± 0.1 | - | - | - | - | |||
| 18 | 239, 263, 343 | 935/933 | 771(100); 609(10); 285(5) | K-3-O-caffeoyldiglucoside- 7-O-glucoside |
- | - | - | 29.4 ± 0.3 | 39.0 ± 5.8 | 18.6 ± 0.3 | - | - | - | - | |||
| 19 | 239, 253, 353 | 965/963 | 801(100); 625(9); 301(3) | Q-3-O-feruloyldiglucoside- 7-O-glucoside |
- | - | - | 12.6 ± 0.1 | 31.9 ± 4.8 | 10.6 ± 0.2 | - | - | - | - | |||
| 20 | 239, 269 sh, 329 | 1495/1493 | 1169(100); 609(39); 285(7) | K-3-O-sinapoylhydroxyferuloyl triglucoside-7-O-diglucoside | 10.4 ± 0.3 | 20.0 ± 2.1 | - | 19.1 ± 0.2 | 20.0 ± 2.9 | 11.1 ± 0.0 | - | - | - | - | |||
| 21 | 239, 269 sh, 330 | 1465/1463 | 1139(100); 609(21); 285(3) | K-3-O-feruloylhydroxyferuloyl triglucoside-7-O-diglucoside | 5.6 ± 0.2 | 31.3 ± 2.7 | 30.7 ± 1.8 | - | - | - | - | - | - | - | |||
| 22 | 239, 267, 331 | 1509/1507 | 1301(7); 1183(100); 609(27); 285(5) | K-3-O-disinapoyltriglucoside- 7-O-diglucoside |
22.4 ± 1.3 | 53.0 ± 2.7 | 18.2 ± 1.5 | 18.9 ± 0.3 | 24.0 ± 3.8 | 16.2 ± 1.8 | - | - | - | - | |||
| 23 | 239, 267 sh, 327 | 1479/1477 | 1153(100); 947(25); 609(33); 285(4) | K-3-O-feruloylsinapoyl triglucoside-7-O-diglucoside |
16.6 ± 0.4 | 37.7 ± 3.0 | 10.9 ± 1.3 | - | - | - | - | - | - | - | |||
| 24 | 240, 331 | 777 b/753 | 529(100) | 1,2-disinapoyl gentiobioside | 57.2 ± 0.0 | 51.3 ± 2.4 | - | - | - | - | 27.8 ± 0.7 | 50.6 ± 2.4 | 39.4 ± 3.7 | 17.1 ± 0.2 | |||
| 25 | 240, 327 | 747 b/723 | 529(38); 499(100) | 1-sinapoyl-2-feruloyl gentiobioside | 55.6 ± 0.7 | 55.6 ± 1.3 | - | - | - | - | 15.5 ± 0.1 | 36.9 ± 3.6 | 48.7 ± 3.9 | 33.3 ± 0.7 | |||
| 26 | 239, 294 sh, 327 | 717 b/693 | 499(100) | 1,2-diferuloyl gentiobioside | 9.0 ± 0.0 | 1.6 ± 0.8 | - | - | - | - | 0.94 ± 0.0 | 0.7 ± 0.0 | 45.4 ± 4.2 | 32.4 ± 0.8 | |||
| 27 | 240, 327 | 983 b/959 | 735(100); 511(21) | 1,2,2′-trisinapoyl gentiobioside | 20.9 ± 0.7 | 5.4 ± 1.2 | 8.9 ± 0.7 | 34.5 ± 0.5 | 28.1 ± 4.0 | 18.3 ± 0.3 | 55.1 ± 3.2 | 19.5 ± 0.9 | 45.7 ± 4.2 | 66.3 ± 1.8 | |||
| 28 | 239, 327 | 953 b/929 | 735(98); 705(100); 511(13); 481(7) | 1,2′-disinapoyl-2-feruloyl gentiobioside | 14.8 ± 0.1 | 0.98 ± 0.0 | 1.3 ± 0.1 | 3.4 ± 0.04 | 7.6 ± 1.1 | 0.75 ± 0.0 | 33.7 ± 0.5 | 12.8 ± 2.5 | 8.3 ± 0.7 | 5.2 ± 0.5 | |||
aK, kaempferol; Q, quercetin. b[M + Na]+ for gentiobiosides.