TMEM158 expression using data downloaded from TCGA database. (A) TMEM158 expression was assessed in laryngeal carcinoma tissues and adjacent normal tissues using mRNA sequencing data from the TCGA database. Red represents cancer tissues and blue represents adjacent normal tissues. ***P<0.001, normal tissue vs. laryngeal cancer. (B) TMEM158 expression at different tumor-node-metastasis stages. *P<0.05 and ***P<0.001, stage I/II/III/IV vs. normal. (C) Kaplan-Meier survival analysis of patients with laryngeal cancer based on TMEM158 expression. ***P<0.001, lowTMEM158 group vs. highTMEM158 group. (D) Reverse transcription-quantitative PCR was performed to assess TMEM158 mRNA expression in human laryngeal squamous cell carcinoma cells and NP69 nasopharyngeal epithelial cells. **P<0.01, LSC-1 cells vs. NP69 cells. TMEM158, transmembrane protein 158; TCGA, The Cancer Genome Atlas.