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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Mol Carcinog. 2020 May 11;59(8):955–966. doi: 10.1002/mc.23214

Figure 3:

Figure 3:

Effect of histone deacetylase inhibitors on maspin expression in prostate cancer cells. (A) Human prostate cancer LNCaP and (B) DU145 cells treated with indicated concentrations of NaB and TSA for 48 h. Total cell lysates, cytosolic and nuclear fractions were prepared, electrophoresed by SDS‐PAGE, followed by immunoblotting with anti-maspin and anti-β-actin and Total histone H3 antibodies. β-actin and histone H3 serves as loading controls. HDAC activity was measured in these cells. Bars ± SD of three independent assays. *p < 0.05, **p < 0.001, compared to control.