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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Mol Carcinog. 2020 May 11;59(8):955–966. doi: 10.1002/mc.23214

Figure 5:

Figure 5:

Effect of class I HDAC knockdown on maspin and p53 expression. (A) LNCaP cells were individually silenced for HDAC 1, 2, 3, and 8. The lysates were prepared, electrophoresed by SDS‐PAGE, followed by immunoblotting with anti-HDAC 1, 2, 3 and 8, anti-maspin and anti-p53 antibodies. GAPDH serves as loading control. (B) Network analysis of maspin (SERPINB5) showing biological binding and expression associated with SERPINB5. (C) Class I HDAC silenced LNCaP cells were immunoprecipitated with maspin or p53. The association was ascertained by immunoblotting for p53 in maspin pulldown lysates and the vice versa for maspin expression. (D) Chromatin immunoprecipitation assay was performed to analyze the binding of p53 to the maspin promoter in the HDAC silenced LNCaP cells.