FIGURE 1.
Variation in donor‐specific cytokine release in PBMC‐NSG mice. Irradiated (100 cGy) NSG mice were injected IV with PBMC (20 × 106 cells) from 10 donors (4692, 4625, 4668, 362, 366, 345, 213, 364, 353, 309). On day 6 post‐engraftment, PBMC‐NSG mice were treated IV with PBS, OKT3 (0.5 mg/kg), or anti‐CD28 (1 mg/kg) as described in the Materials and Methods. Six hours after treatment sera were collected for human cytokine analysis including, (A) IFN‐gamma, (B) IL10, (C) IL6, (D) IL2, (E) IL4, and (F) TNF. Cytokine levels (pg/mL) ± SEM are shown. Each experimental group represents three to five mice. *P < .05, **P < .01, ***P < .001, ****P < .0001