FIGURE 4.
Donor‐specific cytokine release in PBMC‐NSG mice is reproducible between experiments. PBMC from donor 213 were used to setup five independent experiments on different days. Irradiated NSG mice were injected IV with PBMC (20 × 106 to 30 × 106 cells) from donors 213. Day 6 post engraftment mice were treated IV with either PBS, OKT3 (0.5 mg/mL), anti‐CD28 (0.2 mg/mL) in one experiment, or in a second experiment, with PBS, OKT3 (0.1 mg/mL), or Keytruda (5 to 10 mg/kg) as described in the Materials and Methods. Six hours after treatment serum was collected for human cytokine analysis including, (A) IFN‐gamma, (B) IL10, (C) IL6, and (D) TNF. Cytokine levels (pg/mL) ± SEM are shown. Each experimental group represents five to five mice. *P < .05, **P < .01, ***P < .001, ****P < .0001