FIGURE 5.
Stimulation of cytokine release by TGN1412 in PBMC‐SGM3 mice. NSG‐SGM3 mice were irradiated and then injected with 15 × 106 PBMC from donor 3251. Six days after injection PBMC‐NSG mice were treated IV with PBS, anti‐CD28 (1 mg/kg), or TGN1412 analog (0.5, 1.0, or 2.0 mg/kg). Serum samples were collected for human cytokine analysis at 1, 2, 4, and 6 hours posttreatment. Cytokine quantification included; (A) IFN‐gamma, (B) IL10 and (C) IL6, (D) IL2, (E) IL4, and (F) TNF. The results are representative of two independent experiments. Cytokine levels (pg/mL) ± SEM are shown. Each experimental group represents four mice. *P < .05, **P < .01, ***P < .001, ****P < .0001