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. 2020 Jul 27;15:5299–5315. doi: 10.2147/IJN.S250367

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© 2020 Qin et al.

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Pulmonary exposure to ZnONPs caused the reduced number of neurons in mouse cerebral cortex. After a single treatment of 3, 6, 12 μg/animal ZnONPs, cerebral cortex tissues were collected for immunofluorescence assay at post-exposure day 3. (A–C) Representative images obtained from immunofluorescence reflecting NeuN, MAP2 and Ki67 expressions in mouse cortex tissue. Average fluorescence intensities of NeuN, MAP2 and Ki67 were analyzed by Image-Pro Plus image analysis. Scale bar = 100 μm in original figures or 50 μm in zoom-in figures. Data were derived from at least three independent experiments and were reported as mean ± SD. *Denoted P< 0.05, compared with the vehicle control.