FIGURE 3.

Effect of Δ⁹‐tetrahydrocannabinol (THC) on the transcriptional profiles of gene expression in the pulmonary epithelial cells from staphylococcal enterotoxin‐B (SEB)‐induced acute respiratory distress syndrome (ARDS). Lung epithelial cells were isolated from mice which were challenged with SEB followed by THC or vehicle as described in the Figure 1 legend. The differences of gene expression between THC‐treated group and vehicle control group were shown in the scatter plot (a). The expression differences of selected genes between THC‐treated group and vehicle control group were presented in the heat map (b). Quantitative validations of the differential expressions of genes have been presented, including lysozyme1, lysozyme2, β‐defensin‐1 and β‐defensin‐2 (c); claudin, Zo‐1 and occludin‐1 (d); and Mucin2 and Muc5b (e). Data were presented as mean ± SEM of multiple experiments from five mice in each group. P‐value of <0.05 was considered statistically significant, ^* P < 0.05