Figure 6.

Histological lung injury score. Hematoxylin/eosin‐stained 0.6 µm‐thick sections from anterior and posterior lung tissue blocks from the inflation‐fixed right lung of each animal were scored for presence of [a] neutrophils in alveolar space; [b] neutrophils in interstitial space; [c] hyaline membranes; [d] proteinaceous debris in airspaces; and [e] alveolar septal thickening, and reported as a composite histological lung injury score = [(20 × a) + (14 × b) + (7 × c) + (7 × d) + (2 × e)]/(number of fields × 100) (A). (N = 5; ***P < .001 compared with the control group). Representative images from anterior and posterior tissue sections shown (B). Ultrasound score calculated for right lung compared with (C) right lung composite histology score and (D) calculated intrapulmonary shunt, by linear regression with 95% confidence interval denoted by the dashed line (both slopes indicate significant correlation, P < .01) [Color figure can be viewed at wileyonlinelibrary.com]