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. 2020 Aug 3;20(4):80. doi: 10.3892/ol.2020.11941

Figure 3.

Figure 3.

HNF1β is a target of miR-375-3p in LSCC cells. (A) The predicted binding sites of miR-375-3p at the 3′-UTR of HNF1β. Overexpression of miR-375-3p decreased the luciferase activity of (B) AMC-HN-8 and (C) Tu-212 cells expressing WT but not MUT 3′-UTR of HNF1β. miR-375-3p knockdown increased the luciferase activity of 3′-UTR of HNF1β in (D) AMC-HN-8 and (E) Tu-212 cells. (F) Overexpression of miR-375-3p inhibited the mRNA level of HNF1β, while miR-375-3p knockdown increased mRNA expression of HNF1β in LSCC cells. (G) Overexpression of miR-375-3p reduced the protein level of HNF1β and depletion of miR-375-3p increased the protein abundance of HNF1β. ***P<0.001 vs. control miRNA. HNF1β, hepatocyte nuclear factor 1β; miR, microRNA; LSCC, laryngeal squamous cell carcinoma; UTR, untranslated region; WT, wild-type; MUT, mutant.