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. 2020 Aug 3;20(4):80. doi: 10.3892/ol.2020.11941

Figure 4.

Figure 4.

miR-375-3p regulates glucose metabolism of LSCC cells. (A) Overexpression of miR-375-3p decreased the glucose uptake of LSCC cells, while miR-375-3p knockdown increased glucose metabolism of LSCC cells. (B) Overexpressed miR-375-3p decreased the lactate production and inhibition of miR-375-3p increased the lactate generation of LSCC cells. (C) Western blot analysis was performed to assess HNF1β protein expression in AMC-HN-8 and Tu-212 cells transfected with Flag-vector or Flag-HNF1β. (D) Restoration of HNF1β significantly reversed the suppressive role of miR-375-3p in the glucose consumption LSCC cells. (E) Transfection of HNF1β attenuated the decreased lactate production of LSCC cells induced by miR-375-3p. (F) Overexpression of HNF1β significantly reversed the inhibitory effect of miR-375-3p on the proliferation of AMC-HN-8 cells. (G) Restoration of HNF1β reversed the suppressive effect of miR-375-3p on the proliferation of Tu-212 cells. **P<0.01 and ***P<0.001 vs. control miRNA. miR, microRNA; LSCC, laryngeal squamous cell carcinoma; HNF1β, hepatocyte nuclear factor 1β; OD, optical density.