Figure 3.

Combination treatment downregulates mRNA and protein levels of cFLIP and survivin. Reverse transcription-quantitative PCR was used to assess the mRNA expression levels of (A) cFLIP, (B) DR5 and (C) survivin following treatment with 25 ng/ml TRAIL and 25 nM YM155 for 24 h. Data are presented as the mean and standard deviation of three independent experiments. One-way ANOVA followed by Tukey's post hoc test was used, and P-values are indicated. (D) Western blotting was performed to assess protein expression levels of cFLIP, DR5 and survivin after treatment with 25 ng/ml TRAIL and 25 nM YM155 for 24 h. GAPDH and β-tubulin were used as the loading controls. cFLIP, CASP8 and FADD like apoptosis regulator; DR5, death receptor 5; TRAIL, tumor necrosis factor-related apoptosis inducing ligand.