Figure 2.

Triclosan induces mitochondrial morphology and function changes in A375 cells. (A) Triclosan dose-dependently decreased the MMP of A375 cells after 24 h treatment. Statistical significance was determined using one-way ANOVA followed by Tukey's post hoc test. Scale bar, 50 µm. (B) Analyzed data of ATP content indicated that 20 µM triclosan decreased ATP in A375 cells after 24 h. Statistical significance was determined using an unpaired Student's t-test. (C) Acute treatment of 20 µM triclosan increased the mito-ROS levels, caused mitochondrial swelling and mitochondrial fission. Scale bar, 10 µm. (D) Enlarged images of the box area showed mitochondrial swelling and mitochondrial fission after 24 h treatment. Scale bar, 10 µm. (E) Triclosan (20 µM) increased the intracellular ROS levels after 24 h treatment. Scale bar, 50 µm. **P<0.01 vs. 0 µM or control. mito-ROS, mitochondrial reactive oxygen species; MMP, mitochondrial membrane potential.