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. 2020 Aug 18;19:295. doi: 10.1186/s12936-020-03365-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Effects of the amounts of 3D7 DNA and template copy number on C_t values. 3D7 P. falciparum DNA standards were tested by 2-way ANOVA for the effect of template copy number on the C_t value, which decreased as the amounts of parasite DNA and template copy numbers increased (mean C_t values and standard deviations are provided for 3D7 P. falciparum DNA from 10^° to 10⁺³ pg per qPCR, p < 0.001)