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. 2020 Aug 18;19:295. doi: 10.1186/s12936-020-03365-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Limits of detection for 3D7 and Dd2 P. falciparum DNA by template copy number. The LoDs of qPCR assays for drug-susceptible (3D7) and –resistant (Dd2) P. falciparum DNA decreased (analytical sensitivities increased) as the template copy numbers of the qPCR assays increased (p < 0.001). Note that the TARE-2 assay (n = 160) yielded consistently positive results for the lowest concentration of Dd2 DNA tested initially (10⁻⁴ pg DNA per qPCR) but did not yield consistently positive results for an additional dilution of Dd2 DNA (10⁻⁵ pg DNA per qPCR)