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. 2020 Aug 18;20:400. doi: 10.1186/s12935-020-01492-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — MiR-578 overexpression hindered BC progression in vitro. a qRT-PCR for miR-578 expression in 70 pairs of tumor tissues and adjacent normal tissues. b Correlation between miR-578 expression and circZFR level in BC tissues using the Spearman test. c MiR-578 expression by qRT-PCR in MCF10A, MCF7, BT-549, MDA-MB-231 and MDA-MB-453 cell lines. MCF7 and BT-549 cell lines were transfected with miR-NC mimic or miR-578 mimic, followed by the determination of cell viability by CCK-8 assay (d), colony formation using a standard colony formation assay (e), cell apoptosis by flow cytometry (f), cell migration (g) and invasion (h) by transwell assay, glucose uptake, lactate product and ATP level using assay kits (i–k). *P < 0.05