Fig. 7.

CircZFR modulated HIF1A expression via sponging miR-578. a Schematic of the putative miR-578-binding sequence and mutated the target sequence. b, c Relative luciferase activity in MCF7 and BT-549 cell lines cotransfected with HIF1A-3′UTR-WT or HIF1A-3′UTR-MUT and miR-578 mimic or miR-NC mimic. HIF1A mRNA and protein levels by qRT-PCR and western blot in MCF7 and BT-549 cell lines transfected with miR-578 mimic or miR-NC mimic (d, e), sh-NC-infected or sh-circZFR-transduced MCF7 and BT-549 cell lines transfected with anti-NC or anti-miR-578 (f, g). h Relative HIF1A mRNA expression by qRT-PCR in 70 pairs of tumor tissues and adjacent normal tissues. i Correlation between HIF1A expression with circZFR or miR-578 level in BC tissues using Spearman test. *P < 0.05