Table 2.
Limited pollination does not restore male transmission in pigs-1 or gpi8–2
| Observed No. of progeny | ||||||
|---|---|---|---|---|---|---|
| Female parent | Male parent | BastaRa | BastaSa | TE (R/S) | Χ2 | P-value |
| Wild type | pigs-1/+ | 3 | 121 | 0.02 | 112.3 | < 0.001 |
| Wild type | gpi8–2/+ | 1 | 150 | 0.01 | 147.0 | < 0.001 |
| pigs-1/+ | Wild type | 51 | 71 | 0.72 | 3.28 | 0.07 |
| gpi8–2/+ | Wild type | 47 | 63 | 0.75 | 2.33 | 0.13 |
TE, Transmission efficiency was calculated as the quotient of basta resistant (R) divided by susceptible (S) progeny of the indicated cross in which only a limited number of pollen grains (< 40) were used
χ2 was calculated based on the expectation of a 1:1 segregation of basta resistance to susceptibility in the progeny
aBasta herbicide resistant (BastaR) or susceptible (BastaS) progeny. Basta resistance gene is linked with the T-DNA that is inserted into the GPI8 or PIGS genes (pigs-1 and gpi8–2 mutants, respectively)