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. 2020 Aug 18;20:380. doi: 10.1186/s12870-020-02587-x

Table 3.

Seedling lethality of pigs-1/pigs-1; pPIGS:GFP-PIGS on MS plates

Selfed parent Observed No. of progeny % Lethal
Normal Lethal
pigs-1/+ 346 0 0
pigs-1/+; GFP-PIGS #15–3 315 78 19.85
pigs-1/+; GFP-PIGS #7–25 140 32 18.60
pigs-1/+; GFP-PIGS #25–6 324 127 28.16

Selfed seeds were placed onto MS plates containing no antibiotic or basta

Seedling phenotypes were scored according to images and descriptions in Fig. 3

GFP-PIGS refers to three independent transformant T2 lines of pPIGS:GFP-PIGS