Table 4.

Transmission of pigs-1 mutation in self-pollinated pistils

Selfed parent	Observed No. of progeny
	BastaR	BastaS	Lethal	Χ2	P-value
+/+; BastaR/+	130	43	0	0.001	0.97
pigs-1/+	151	153	0	52.01	< 0.001
pigs-1/+; GFP-PIGS #15–3	512	256	166	101.4	< 0.001
pigs-1/+; GFP-PIGS #7–25	152	74	65	40.22	< 0.001
pigs-1/+; GFP-PIGS #25–6	402	154	112	44.61	< 0.001

Basta herbicide-resistant (Basta^R) or susceptible (Basta^S) progeny. Basta resistance is linked with the T-DNA that is inserted into either the AtPIGS gene (pigs-1 mutant) (Fig. 2a) or in an unknown location in the genome of the +/+; BastaR/+ (positive control line) [21]

χ2 was calculated based on the expectation of a 3:1 segregation of basta resistance to susceptibility in the progeny

Seedling phenotypes scored according to images and descriptions in Fig. 3

GFP-PIGS refers to three independent transformant T2 lines of pPIGS:GFP-PIGS