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. 2020 Aug 18;20:381. doi: 10.1186/s12870-020-02593-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — OsCAF2 interacts with OsCRS2 through the C-terminal region. a The Y2H interaction between OsCAF2 and OsCRS2. The full-length CDS of OsCAF2 and OsCRS2 were cloned into pGBKT7 (BD) and pGADT7 (AD) vectors to produce OsCAF2-BD, OsCAF2-AD, OsCRS2-BD, and OsCRS2-AD plasmids, respectively. The OsCAF2-BD and OsCRS2-AD, OsCAF2-AD and OsCRS2-BD were co-transformed into the AH109 yeast strain. The OsCAF2-BD and AD plasmid, and OsCRS2-AD and BD plasmid were co-transformed into the AH109 yeast strain as a control. b Schematic diagram of OsCAF2 protein structure. Different sections of OsCAF2 involving in OsCAF2-N (1–230 aa), OsCAF2-M (231–430 aa), and OsCAF2-C (431–608 aa). c Y2H interaction of OsCRS2 and different truncated sections of OsCAF2. The truncated sections of OsCAF2 were cloned into the BD vector to generate OsCAF2-N-BD, OsCAF2-M-BD, and OsCAF2-C-BD vectors. These three vectors were co-transferred into AH109 yeast cells with the OsCRS2-AD vector. The yeast cells were cultured on SD-T/L and SD−/T/L/H/A media with different dilution series (1, 10^− 1, and 10^− 2)