Figure 3.

TMAO treated slices exhibits alterations in basal synaptic transmission and paired-pulse facilitation (PPF). For (A–C), hippocampal slices were incubated in ACSF with either 0.03% DMSO (vehicle) or TMAO (50 μM) for 4 h before recording. (A) The input-output curve of the fEPSP slope measured at increasing stimulus intensities. (B) The input-output curve of FV amplitude measured at increasing stimulus intensities. (C) Change in the ratio of second stimulus fEPSP (EPSP2) to the first stimulus fEPSP (EPSP1) slope plotted as a function of interstimulus interval. (D) Representative western blot showing vGLUT1 and synaptophysin relative densities normalized to GAPDH in slices treated with either vehicle or TMAO (50 μM); for panels (A–C) n = 5–6 slices from four mice per group; for (D) n = 3 mice per group; for panels (A–C) planned pairwise comparisons were performed for individual data point analysis for a vehicle vs. TMAO-treated slices. Symbols/bars represent mean ± SEM; *indicates significant difference between vehicle vs. TMAO-treated slices, *p < 0.05, **p < 0.001.