Table 1.
Summary of antibodies and working conditions used in the experiments.
| Antibodies | Species | Source | Catalog# | Dilution |
|---|---|---|---|---|
| Primary antibodies | ||||
| ATF-4 | Rabbit | Cell Signaling Technology | 11815 | 1:1,000 |
| CHOP | Mouse | Cell Signaling Technology | 2895 | 1:1,000 |
| CREB | Mouse | Cell Signaling Technology | 4820 | 1:1,000 |
| Phospho-CREB | Rabbit | Cell Signaling Technology | 9198 | 1:1,000 |
| eIF2α | Rabbit | Cell Signaling Technology | 5324 | 1:1,000 |
| Phospho-eIF2α (Ser51) | Rabbit | Cell Signaling Technology | 3398 | 1:1,000 |
| FMO3 | Rabbit | Abcam | 126711 | 1:1,000 |
| GAPDH | Rabbit | Cell Signaling Technology | 5174 | 1:1,000 |
| GluA1 | Rabbit | Cell Signaling Technology | 13185 | 1:1,000 |
| GluA2 | Rabbit | Cell Signaling Technology | 13607 | 1:1,000 |
| GluN2A | Rabbit | Cell Signaling Technology | 4205 | 1:1,000 |
| GluN2B | Rabbit | Cell Signaling Technology | 14544 | 1:1,000 |
| PERK | Rabbit | Cell Signaling Technology | 5683 | 1:1,000 |
| phospho-PERK | Rabbit | Cell Signaling Technology | 3179 | 1:1,000 |
| PSD95 | Rabbit | Cell Signaling Technology | 3450 | 1:1,000 |
| Synaptophysin | Rabbit | Cell Signaling Technology | 36406 | 1:1,000 |
| α-tubulin | Mouse | DSHB | 12G10 | 1:1,000 |
| vGLUT1 | Rabbit | Cell Signaling Technology | 12331 | 1:1,000 |
| Secondary antibodies | ||||
| Anti-mouse IgG | Horse | Cell Signaling Technology | 7074P2 | 1:5,000 |
| Anti-rabbit IgG | Goat | Cell Signaling Technology | 7076P2 | 1:5,000 |