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. 2020 Aug 9;26:e924670-1–e924670-12. doi: 10.12659/MSM.924670

Figure 4.

Figure 4

HIF-1α was required in DAAM2-induced invasion of HCC. (A) MG132 (10μM) incubation for 6 hr had no influence on proliferation of HuH7. Proliferation was assessed by MTT assay. (B) Invasion of HuH7 was detected after overexpressing DAAM2. 10 μM MG132 or 10 μM HIF-1α inhibitor BAY87-2243 was used to incubate HuH7. ** Represented P<0.01 compared with control group, and ## represented P<0.01 between indicated groups. Analyzed data were from three independent experiments. (C) Representative images in transwell assay of B. (D) Western blot showed that HIF-1α was knocked down while DAAM2 was overexpressed in HuH7. (E) HIF-1α knockdown extensively decreased the DAAM2-induced invasion. The invasion was detected with transwell assay. ## Represented P<0.01 between indicated groups. (F) Representative images in transwell assay of (E).