Table 3.
Correlation between p53 Protein Expression (by IHC) and mRNA Expression of p53 and Its Seven Target Genes in CCA Tissue Samples
| mRNA expression | p53 protein# | mRNA | p53 protein# | P-value* | |||||
|---|---|---|---|---|---|---|---|---|---|
| n | Low expression 9 (30%) |
High expression 21 (70%) |
P-value* | expression | n | Low expression 9 (30%) |
High expression 21 (70%) |
||
| (n = 30) | (n = 30) | ||||||||
| p53 | PAI-1 | ||||||||
| Down-regulated | 7 | 1 | 6 | 0.829 | Down-regulated | 29 | 9 | 20 | 1.000 |
| Unchanged | 20 | 7 | 13 | Unchanged | 1 | 0 | 1 | ||
| Up-regulated | 3 | 1 | 2 | Up-regulated | 0 | 0 | 0 | ||
| p21 | FUCA1 | ||||||||
| Down-regulated | 15 | 3 | 12 | 0.204 | Down-regulated | 14 | 6 | 8 | 0.236 |
| Unchanged | 14 | 5 | 9 | Unchanged | 16 | 3 | 13 | ||
| Up-regulated | 1 | 1 | 0 | Up-regulated | 0 | 0 | 0 | ||
| MDM2 | S100A9 | ||||||||
| Down-regulated | 14 | 3 | 11 | 0.580 | Down-regulated | 14 | 6 | 8 | 0.376 |
| Unchanged | 14 | 5 | 9 | Unchanged | 14 | 3 | 11 | ||
| Up-regulated | 2 | 1 | 1 | Up-regulated | 2 | 0 | 2 | ||
| WIP1 | ICAM2 | ||||||||
| Down-regulated | 11 | 0 | 11 | 0.007* | Down-regulated | 17 | 4 | 13 | 0.596 |
| Unchanged | 16 | 7 | 9 | Unchanged | 12 | 5 | 7 | ||
| Up-regulated | 3 | 2 | 1 | Up-regulated | 1 | 0 | 1 | ||
#p53 protein with IHC score either in nucleus or in cytoplasm higher than median were classified as high p53, and those with IHC score both in nucleus and cytoplasm lower than median were low p53; *The comparison of variables that had 3 categories, significance at ≤ 0.05 using Fisher’s exact probability test.