(A) Domain organization of KRAS, neurofibromin (NF1), and SPRED1 showing the presence of various domains in the full-length protein. The red line drawn above each protein indicates the domains involved in complex formation and used for our structural studies. In NF1, the GAPc and GAPex regions are highlighted in light and dark green, respectively.
(B) The overall structure of the complex formed by GMPPNP-bound KRAS (light brown), NF1(GRD) (light green), and SPRED1(EVH1) (light pink) proteins. In the left panel, the ternary complex is indicated in ribbon representation, whereas in the right panel, proteins are indicated in surface representation. In both panels, GMPPNP and Mg2+ bound to KRAS are indicated as sticks and spheres, respectively.
See also Figure S1 and Table S1.