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. 2020 Jun 2;245(14):1233–1241. doi: 10.1177/1535370220929965

Table 3.

Quantitative polymerase chain reaction primers and products sizes.

Amplicon Primers Best transcript Gene symbol Product length
hsa_circ_101622 F: 5'CAGATGGACAAAGTGGAGCA3' NM_18689 CEMIP 131bp
R: 5'GCTGGGGAAGTCATTCTTGA3'
hsa_circ_022382 F: 5'GGATGGCTGCAACATGATTA3' NM_004265 FADS2 157bp
R: 5'TCAGCAGGGGTTTCAAGAAC3'
hsa_circ_100833 F: 5'GCCAAGCCTAACATCTTCCA3' NM_004265 FADS2 145bp
R: 5'TCAGCAGGGGTTTCAAGAAC3'
hsa_circ_101621 F: 5'ACCCACCCACATACATCAGG3' NM_018689 CEMIP 132bp
R: 5'ATGTCCACGCCGTCTATCTC3'
hsa_circ_008068 F: 5'TGATGAAGCTTTGCGAAGAA3' NM_032116 KATNAL1 137bp
R: 5'CGTAATTTCCAAGAAGGGCATA3'
hsa_circ_051749 F: 5'TCATCTTCCTCGCCAGCTAC3' NM_000836 GRIN2D 142bp
R: 5'AGTGAGGCACATGACGAACA3'
hsa_circ_407279 F: 5'CCCAGTGGAGGCTGTTCTAC3' NM_138780 SYTL5 130bp
R: 5'AAGATTCCCCCAAGGAATTC3'
hsa_circ_406503 F: 5'AGTTGTGGGGTGGTCCTCTG3' NR_037884 LOC100507053 170bp
R: 5'TAGCAGAAAACTGCGGGTCT3'
hsa_circ_406549 F: 5'CTGGTTCCTCAGCTCTCCAC3' NM_000901 NR3C2 169bp
R: 5'GGATCATCTGTTTGCCTGCT3'
hsa_circ_406015 F: 5'CGGACAGCCTCTCAGTCTCT3' NM_015265 SATB2 197bp
R: 5'GTTGTCGGTGTCGAGGTTTT3'
GAPDH mRNA F: 5'GGGAAACTGTGGCGTGAT3' NM_001289746 GAPDH 299bp
R: 5'GAGTGGGTGTCGCTGTTGAC3'
FADS2 mRNA F: 5'ACGCTGGAGAAGATG3' NM_004265 FADS2 307bp
R: 5'GCCGTGATGAGGGTAG3'

F: forward; R: reverse; Chr: chromosome; bp: base pairs.