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. 2020 Jun 9;245(14):1254–1259. doi: 10.1177/1535370220931043

Figure 1.

Figure 1.

Oral intake of LPS induces feedback-granulopoiesis independent of G-CSF. (a–d) C57BL/6 mice were treated for 11 days with LPS-hi or LPS-RS in the drinking water (both 5 mg/L). (a) Blood was obtained at d11 and analyzed by flow cytometry. Neutrophil numbers were calculated according to the white blood count obtained with an Advia blood analyzer (n = 3 per group). (b) G-CSF levels in the sera of LPS-hi (n = 3), LPS-RS (n = 7) or untreated mice (n = 6) were analyzed at d11 by ELISA. (c–d) On d3 of the experiment, mice were additionally treated with an αLy-6G mAb (1A8, 1.25 mg/mouse i.p.) or vehicle control every 48 h and the bone marrow was analyzed at d11 by flow cytometry. (c) Exemplary hierarchical gating scheme of GMPs (CD16/32+CD34+), CMPs (CD16/32loCD34+), and MEPs (CD16/32−CD34−) within the Lin−c-kit+Sca-1− population and LSKs (Lin−c-kit+Sca-1+) of untreated mice receiving vehicle control or αLy-6G mAb. (d) On d11, bone marrow progenitor cells were analyzed in mice treated with LPS-hi (n = 3), LPS-RS (n = 4) or untreated controls (n = 3) by flow cytometry. (e–f) C57BL/6 mice were treated for three days with LPS-hi (5 mg/L) in the drinking water or left untreated. In addition, αG-CSF mAb (10 µg/mouse every 24 h i.p.) was injected to deplete G-CSF and mice were analyzed at d3. (e) G-CSF levels in the sera were analyzed by ELISA (n = 4 per group). (f) Bone marrow (n = 3 per group) was analyzed for LSK, GMP, CMP and MEP populations by flow cytometry.