Figure 2.

Oral LPS is sensitized by TLR4 signalling. (a–b) TLR4^−/− mice were treated for 11 days with LPS-hi (5 mg/L) in the drinking water or left untreated. On d3 of the experiment, mice were additionally treated with an αLy-6G mAb (1A8, 1.25 mg/mouse i.p.) or vehicle control every 48 h and the bone marrow was analyzed at d11 by flow cytometry. (a) Blood was obtained at d11 and analyzed by flow cytometry. Neutrophil numbers were calculated according to the white blood count obtained with an Advia blood analyzer (n = 7 per group). (b) On d11, bone marrow progenitor cells were analyzed in mice treated with LPS-hi (n = 3) and untreated controls (n = 5) by flow cytometry. (c–e) Mice of different conditional TLR4 knockout strains were treated with an αLy-6G mAb (1A8, 1.25 mg/mouse i.p.) or vehicle control every 48 h and analyzed at d8. (c) Bone marrow of TLR4^fl/fl and TLR4^fl/flVav^cre mice was analyzed for progenitor cell populations (n = 8 per group) by flow cytometry (left) and G-CSF levels in the sera were measured by ELISA (n = 10 per group) (right). (d) Bone marrow of TLR4^fl/fl and TLR4^fl/flTie2^cre mice was analyzed for progenitor cell populations (n = 4 per group) by flow cytometry (left) and G-CSF levels in the sera were measured by ELISA (n = 4 per group) (right). (e) Bone marrow of TLR4^fl/fl (Ctrl. n = 5; αLy-6G n = 4) and TLR4^fl/flNes^cre (n = 5 per group) mice was analyzed for progenitor cell populations by flow cytometry (left) and G-CSF levels in the sera of TLR4^fl/fl (Ctrl. n = 4; αLy-6G n = 3) and TLR4^fl/flNes^cre (n = 5 per group) mice were measured by ELISA (right).