Figure 4.

FOXP3 is a target gene of miR-424. (a) The predicted FOXP3 binding sites with miR-424 &was shown. (b) Two hundred and ninety-three T cells were transfected with luciferase reporter plasmid containing WT or mutant form of FOXP3 and miR-424 mimic or miR-424 inhibitor, the luciferase signal were assessed 24 h later. (c) Relative values of miR-424 expression in NC mimic, miR-424 mimic, NC inhibitor and miR-424 inhibitor was measured by qRT-PCR. (d) FOXP3 expression in cells treated with miR-424 mimic and miR-424 inhibitor was examined by western blot. (e) Relative values of FOXP3 protein expression was analyzed by Image J. (f) Flow cytometric analysis was used to identify the FOXP3-positive cells (P2). Data are shown as mean ± S.E.M. *P < 0.05, **P < 0.01, ***P < 0.001, n = 6. (A color version of this figure is available in the online journal.)