REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
SARS2-01, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-02, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-07, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-11, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-12, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-16, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-18, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-20, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-21, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-22, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-23, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-29, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-31, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-32, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-34, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-38, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-39, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-50, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-55, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-58, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-66, anti-SARS-CoV-2 mAb	This paper	N/A
SARS2-71, anti-SARS-CoV-2 mAb	This paper	N/A
Goat anti-mouse IgG, human ads-HRP	Southern Biotech	Cat # 1030-05; RRID: AB_2619742
CR3022, anti-SARS-CoV-2 mAb	Yuan et al., 2020	N/A
Goat anti-mouse IgG, human ads-BIOT	Southern Biotech	Cat # 1030-08; RRID: AB_2794296
Goat anti-mouse IgA-BIOT	Southern Biotech	Cat # 1040-08; RRID: AB_2794374
Goat anti-mouse IgM-BIOT	Southern Biotech	Cat # 1021-08; RRID: AB_2794242
HRP-conjugated goat anti-human IgG	Thermo Fisher	62-8420; RRID: AB_2533962
MAR1-5A3, anti-Ifnar1 mAb	Leinco	I-401; RRID: AB_2491621
Anti-HuAdV5-hexon antibody	Novus Biologicals	Cat # NB600413; RRID: AB_10002549
Streptavidin-HRP	Vector Laboratories	Cat # SA-5004; RRID: AB_2336509
Peroxidase Streptavidin	Jackson ImmunoResearch	Cat # 016-030-084; RRID: AB_2337238
Alexa Fluor 647 anti-IFNγ	BD BioScience	Cat # 557735; RRID: AB_396843
Anti-mouse CD16/32	eBioSceince	Cat # 14-0161-85; RRID: AB_467134
BUV395 anti-CD45	BD BioSciences	Cat # 564279; RRID: AB_2651134
PE anti-CD44	BioLegend	Cat # 103024; RRID: AB_493687
PerCP/Cy5.5 anti-CD8b	BioLegend	Cat # 126610; RRID: AB_2260149
APC/Cy7 anti-CD19	BioLegend	Cat # 115530; RRID: AB_830707
Fixable Aqua dead cell stain	Invitrogen	Cat # L34965;
BV 605 anti-TNFα	BioLegend	Cat # 506329; RRID:AB_11123912
BV421 anti-CD4	BioLegend	Cat # 100437; RRID: AB_10900241
FITC anti-CD103	BioLegend	Cat # 121419; RRID: AB_10709438
BV711 anti-CD69	BioLegend	Cat # 104537; RRID: AB_2566120
PE anti-granzyme B	Invitrogen	Cat # GRB04; RRID: AB_2536538
PE/Cy5 anti-CD4	BioLegend	Cat # 100409; RRID: AB_312694
Virus and bacterial Strains
SARS-CoV-2 (strain 2019 n-CoV/USA_WA1/2020)	CDC/BEI Resources	NR52281
ChAd-SARS-CoV-2-S	This paper	N/A
ChAd-Control	This paper	N/A
AdV-hACE2	This paper	N/A
E. coli strain BJ5183	Agilent	Cat # 200154
BL21(DE3)-RIL E. coli	Invitrogen	Cat # C601003
Experimental Models: Cell Lines
Vero CCL-81	ATCC	CCL-81; RRID: CVCL_0059
Vero E6	ATCC	CRL-1586; RRID: CVCL_0574
HEK293	ATCC	CRL-1573; RRID: CVCL_0045
HEK293T	ATCC	CRL-3216; RRID: CVCL_0063
Expi293F cells	Invitrogen	Cat # A14527
Experimental Models: Organisms/Strains
Mouse: C57BL/6J	Jackson Laboratory	Cat#000664; RRID: IMSR_JAX:000664
Mouse: BALB/c	Jackson Laboratory	Cat#000651; RRID: IMSR_JAX:000651
Mouse: B6.Cg-Tg(K18-ACE2)2Prlmn/J	Jackson Laboratory	Cat# 034860; RRID: IMSR_JAX:03486
Oligonucleotides
SARS-CoV-2 N F: 5′-ATGCTGCAATCGTGCTACAA-3′	This paper	N/A
SARS-CoV-2 N R: 5′-GACTGCCGCCTCTGCTC-3′	This paper	N/A
SARS-CoV-2 N Probe: 5′-/56-FAM/TCAAGGAAC/ZEN/AACATTGCCAA/3IABkFQ/-3′	This paper	N/A
SARS-CoV-2 ORF1a F: 5′- TTCAGTTGACTTCGCAGTGG −3′	This paper	N/A
SARS-CoV-2 ORF1a R: 5′- GGACGGGTTTGAGTTTTTCA −3′	This paper	N/A
SARS-CoV-2 ORF1a Probe: 5′-/56-FAM/AACTAACAT/ZEN/CTTTGGCACTGTTT/3IABkFQ	This paper	N/A
SARS-CoV-2 RNA ISH probe (S gene)	Advanced Cell Diagnostics	Cat# 4848561
Gapdh TaqMan Primer/Probe set	IDT	Mm.PT.39a.1
Ifng TaqMan Primer/Probe set	IDT	Mm.PT.58.41769240
Il6 TaqMan Primer/Probe set	IDT	Mm.PT.58.10005566
Il1b TaqMan Primer/Probe set	IDT	Mm.PT.58.41616450
Tnfa TaqMan Primer/Probe set	IDT	Mm.PT.58.12575861
Cxcl10 TaqMan Primer/Probe set	IDT	Mm.PT.58.43575827
Ccl2 TaqMan Primer/Probe set	IDT	Mm.PT.58.42151692
Ccl5 TaqMan Primer/Probe set	IDT	Mm.PT.58.43548565
Cxcl11 TaqMan Primer/Probe set	IDT	Mm.PT.58.10773148.g
Ifnb TaqMan Primer/Probe set	IDT	Mm.PT.58.30132453.g
Ifnl(2/3) TaqMan Primer/Probe set	Thermo Fisher	Mm04204156_gH
Software and Algorithms
FlowJo	FlowJo, LLC	v10
GraphPad Prism	GraphPad	v 8.2.1
Biorender	biorender.com	N/A
Recombinant DNA
pABVec6W	Davis et al., 2019	N/A
Simian Ad36 vector	Penn Vector Core	Roy et al., 2011
pSAd36-S	This paper	N/A
pAd36-Control	This paper	N/A
pAd-hACE2	This paper	N/A
AdEasy AdV vector system	Agilent technologies	Cat# 240009
PCR-II topo	Invitrogen	Cat# 451245