KEY RESOURCES BLKEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-UCP-1 | ThermoFisher | Custom made |
| Mouse monoclonal anti-AdoR A2A (7F6-G5-A2) | Santa Cruz | #sc-33261 |
| Goat polyclonal anti AdoR A2B (R-20) | Santa Cruz | #sc-7507 |
| Goat polyclonal anti AdoR A1 (C-19) | Santa Cruz | #sc-7500 |
| Mouse monoclonal anti-Actin (AC-74) | SigmaAldrich | #A2228 |
| Mouse monoclonal anti CD71 (3B8 2A1) | Santa Cruz | sc-32272 |
| Myosin Heavy Chain 1 | DSHB | BA-F8-c |
| Myosin Heavy Chain 2A | DSHB | BF-F3-c |
| Myosin Heavy Chain 2B | DSHB | SC-71-c |
| Bacterial and Virus Strains | ||
| Lentivirus shControl | This study | N/A |
| Lentivirus shA1 | This study | N/A |
| Biological Samples | ||
| Human BAT biopsies | Turku PET Centre, Finland | N/A |
| Human WAT sample collection | University of Leipzig, Germany | approval number: 017-12-23012012 |
| Human SKM samples | University of Cologne and Düsseldorf, Germany | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Bay 60-6583 | Tocris | # 4472 |
| PSB 603 | Tocris | # 3198 |
| CGS 21680 | Tocris | # 1063 |
| A2A TM4 RRRQRRKKRGYAKGIIAICWVLSFAIGLTPMLGW | Biomatik | Custom made |
| A2A TM5 MNYMVYFNFFACVLVPLLLMLGVYL YGRKKRRQRRR | Biomatik | Custom made |
| A2A TM6 RRRQRRKKRGYLAIIVGLFALCWLPLHIINCFTFF |
Biomatik | Custom made |
| A2B TM4 RRRQRRKKRGYARGVIAVLWVLAFGIGLTPFLGW |
Biomatik | Custom made |
| A2B TM5 MSYMVYFNFFGCVLPPLLIMLVIYIYGRKKRRQRRR |
Biomatik | Custom made |
| A2B TM6 RRRQRRKKRGYLAMIVGIFALCWLPVHAVNCVTLF |
Biomatik | Custom made |
| Critical Commercial Assays | ||
| DuoLink PLA Kit | SigmaAldrich | |
| Piccolo General Chemistry 13 | Hitado | 114-400-0029 |
| Piccolo Lipid Plus | Hitado | 114-400-0030 |
| Piccolo Metlyte 8 | Hitado | 114-400-0023 |
| Piccolo Basic Metabolic Plus | Hitado | 114-400-0031 |
| 4-HNE assay | Abcam | ab238538 |
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| C2C12 | ATCC | CRL-1772 |
| Human skeletal muscle cells | Promo Cell | C-12530 |
| Human brown adipocytes | Jespersen et al.; 2013 | N/A |
| hMADS | Rodriguez et al., 2005 | N/A |
| Experimental Models: Organisms/Strains | ||
| Adenosine A2B receptor knockout mouse | Eckle et al., 2007 | N/A |
| HSA-Cre | McCarthy et al. 2012; Jackson Laboratories | Strain 025750 |
| Adipoq-Cre | Jackson Laboratories | Strain 010803 |
| A2Bfloxed | Eckle et al., 2008 | N/A |
| Oligonucleotides | ||
| Gs-coupled GPCR primer | ||
| A2B 5’-gtggaacagtaaagacagtg; 5’-ccatgaagattttgatgtag | This study | N/A |
| Adrb3 5’-gagactacagaccataacc; 5’-atgctagcagttacacag | This study | N/A |
| Drd1 5’-tcaatcagaaagttccttta; 5’-ggcattaaaagcataaataa | This study | N/A |
| Mc4r 5’-tggacaggtatttcactatc; 5’-gaacatgtggacatagagag | This study | N/A |
| Tshr 5’-accaggaatatgaagaagat; 5’-acacaccgtgtagtcatagt | This study | N/A |
| Recombinant DNA | ||
| A2Amut (F257A; F258A) | This study | N/A |
| A2Bmut (L258A; F259A) | This study | N/A |
| Software and Algorithms | ||
| Modeller 9.18 software | Marti-Renom et al. 2000 | https://salilab.org/modeller/ |
| Graph pad prism V7 | Graph pad software | https://graphpad.com |
| Other | ||