Table V.
Comparison of diagnostic value among different microbiological tests. This includes a supposed worst-case scenario analysis including the two patients censored for sequencing failure as either culture-negative prosthetic joint infection (n = 1) or noninfection (n = 1).
| Positive microbiological test | Prosthetic joint infection (n = 51) | Noninfection (n = 21) | Sensitivity, % (95% CI) | Specificity, % (95% CI) | Positive predictive value, % (95% CI) | Negative predictive value, % (95% CI) |
|---|---|---|---|---|---|---|
| Synovial fluid culture | 32 | 0 | 62.7 (49 to 74.7) | 100.0 (84.5 to 100.0) | 100.0 (89.3 to 100.0) | 52.5 (37.5 to 67.1) |
| Comprehensive culture | 41 | 1 | 80.4 (67.5 to 89) | 95.2 (77.3 to 99.2) | 97.6 (87.7 to 99.6) | 66.7 (48.8 to 80.8) |
| Synovial fluid mNGS | 47 | 1 | 92.2 (81.5 to 96.9) | 95.2 (77.3 to 99.2) | 97.9 (89.1 to 99.6) | 83.3 (64.1 to 93.3) |
| Synovial fluid culture or mNGS | 48 | 1 | 94.1 (84.1 to 98.0) | 95.2 (77.3 to 99.2) | 98.0 (89.3 to 99.6) | 87.0 (67.9 to 95.5) |
| Comprehensive culture or synovial fluid mNGS | 51 | 2 | 100.0 (93.0 to 100.0) | 90.5 (71.1 to 97.3) | 96.2 (87.2 to 99.0) | 100.0 (83.2 to 100.0) |
CI, confidence interval; mNGS, metagenomic next-generation sequencing