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PCA and TSNE analyses. (A) The 1000 variant genes screened before were analyzed by PCA, and eventually 20 PCs were obtained. Then (B) the 20 PCs were clustered, ultimately 3 PCs with most significant difference was identified. The abscissa represents empirical value and the vertical axis represents theoretical value. In turn, (C) cells in these 3 PCs were sequentially clustered into four types via TSNE analysis.
