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. 2020 Aug 19;6(34):eaba4897. doi: 10.1126/sciadv.aba4897

Fig. 5. Vps24 interactions including Vps2 and/or Snf7 with POPC/DOPS liposomes at high and low concentrations.

Fig. 5

(A) Flotation (top) and POPC/DOPS liposome flotation (bottom) assays of Vps24, Vps2, and Snf7 alone. At high concentrations (3.0 mg/ml), Vps24 and Vps2 are found to bind liposomes. (B) Flotation (top) and liposome flotation (bottom) assays of Vps24/Snf7 and Vps24/Vps2/Snf7 mixtures. In binary and tertiary high-concentration ESCRT-III complexes, mainly Vps24 and Vps2 can be found to bind liposomes. (C) Negative-stain electron micrographs (top), sketched lipid outlines (middle), and galleries (bottom) of low-concentration Vps24, Vps2/Vps24, and Vps2/Vps24/Snf7 mixtures (0.5 mg/ml) incubated with POPC/DOPS liposomes. Scale bar, 100 nm. Left: Vps24 filaments can be found associated with intact spherical liposomes. Middle: When incubated with Vps2/Vps24, many liposomes have constricting neck structures. Right: The presence of Vps2/Vsp24/Snf7 reveals many tubular membrane structures. (D) Electron cryo-micrographs of low-concentration Vps2/Vps24/Snf7 mixtures confirm the presence of a striated protein coat on POPC/DOPS membrane tubules.