Table 1. Cryo-EM data collection and model refinement statistics.
| Data collection and processing | Vps24 |
| Magnification | ×130,000 |
| Voltage (kV) | 300 |
| Electron exposure (e−/Å2) | 40 |
| Defocus range (μm) | 0.75–3.0 |
| Pixel size (Å) | 1.040 |
| Detector | Gatan K2 Summit |
| Symmetry imposed | C1 and helical |
| Final no. of particle images | 313,554 |
| Helical rise (Å) | 25.18 |
| Helical twist (°) | −32.47 |
| Global map resolution (Å, FSC = 0.143) |
3.2 |
| Local map resolution range (Å) | 2.9–6.0 |
| Model refinement | Vps24 |
| Initial model used (PDB code) | Homology model of PDB ID 3FRT* |
| Model resolution (Å, FSC = 0.143) | 3.0 |
| CC mask† | 0.7972 |
| Map sharpening B-factor (Å2) | −126 |
| Model composition | |
| Nonhydrogen atoms | 4 × 1381 |
| Protein residues | 4 × 170 |
| RMSDs | |
| Bond lengths (Å) | 0.008 |
| Bond angles (°) | 0.914 |
| Validation | |
| MolProbity score | 1.34 |
| Clashscore† | 3.65 |
| EMRinger score† | 1.02 |
| Rotamer outliers (%) | 0.64 |
| Ramachandran plot | |
| Favored (%) | 96.43 |
| Allowed (%) | 2.38 |
| Disallowed (%) | 1.19 |
*(4).
†Computed for a 20-mer.